The Definitive Guide to 2-FDCK HCl
The lab states fixation will cause the dyes to leak from the cells. I do think the assumption is always that fixation could problems cell membranes. I did discover a paper which describes fixation using methanol or acetic acid, just after staining with a DCFDA by-product. DCFDA is definitely the reagent that stains ROS inside the kit ab113851, and